Any feedback?
Literature summary extracted from
- Characterization of the adenosinetriphosphatase activity of the Escherichia coli RecBCD enzyme: relationship of ATP hydrolysis to the unwinding of duplex DNA (1989), Biochemistry, 28, 2873-2881.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.11.5 | NaCl | ATP molecules hydrolyzed per base pair unwound slightly increased | Escherichia coli |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.11.5 | E. coli single stranded DNA binding protein | ATPase activity complete inhibited by SSB | Escherichia coli |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.11.5 | 0.00000013 | - |
Double-stranded DNA | DNA-dependent ATPase activity | Escherichia coli | |
| 3.1.11.5 | 0.085 | - |
ATP | DNA-dependent ATPase activity | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.11.5 | ATP + H2O | Escherichia coli | - |
ADP + phosphate | - |
? | |
| 3.1.11.5 | double-stranded DNA + H2O | Escherichia coli | ATP-dependent helicase | single-stranded DNA fragments | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.11.5 | Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.11.5 | ATP + H2O | - |
Escherichia coli | ADP + phosphate | - |
? | |
| 3.1.11.5 | ATP + H2O | biphasic activity represents DNA unwinding and ATPase activity | Escherichia coli | ADP + phosphate | - |
? | |
| 3.1.11.5 | double-stranded DNA | - |
Escherichia coli | intermediates with single stranded regions | - |
? | |
| 3.1.11.5 | double-stranded DNA + H2O | ATP-dependent helicase | Escherichia coli | single-stranded DNA fragments | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.11.5 | 740 | - |
ATP | DNA-dependent ATPase activity | Escherichia coli | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
